Mkl1,myocardin相关转录因子抗体

产品编号HZ-6973R
英文名称Mkl1
中文名称myocardin相关转录因子抗体
别 名AMKL; Bsac; Mal; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MKL (megakaryoblastic leukemia)/myocardin like 1; MKL/myocardin like protein 1; MKL/myocardin-like protein 1; Mkl1; MKL1_HUMAN; MRTF A; Mrtf A; MRTF-A; MRTFA; MYOCARDIN RELATED PROTEIN; Myocardin related transcription factor A; Myocardin-related transcription factor A.
说 明 书0.1ml 0.2ml
研究领域心血管 细胞生物 信号转导 细胞凋亡 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow,
Mkl1,myocardin相关转录因子抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-F=1:100-500 Flow-Cyt=1:20-200 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45, 90kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Mkl1/MRTF-A
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Mkl1,myocardin相关转录因子抗体PubMedPubMed
产品介绍background:
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.

Function:
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation (By similarity).

Subunit:
Forms with SCAI and SRF a nuclear ternary complex which binds the CArG consensus motif (CArG box) on DNA via SRF. Interacts with ACTB; interaction with ACTB prevents interaction with SCAI. Interacts with MKL2.

Subcellular Location:
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.

Mkl1,myocardin相关转录因子抗体Tissue Specificity:
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.

DISEASE:
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.

Similarity:
Contains 2 RPEL repeats.
Contains 1 SAP domain.

Gene ID:
57591

Database links:
UniProtKB/Swiss-Prot: Q969V6.1

Mkl1,myocardin相关转录因子抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Mkl1/MRTF-A Polyclonal Antibody, Unconjugated(HZ-6973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Mkl1/MRTF-A Polyclonal Antibody, Unconjugated(HZ-6973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Protein: line1,mouse brain;line2,mouse lung; Primary: Anti-Mkl1 (HZ-6973R) at 1:300;  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(HZ-0295G-HRP) at 1: 5000;  ECL excitated the fluorescence;  Predicted band size :45/90 kD Observed band size : 45/90 kD