重组人CD138蛋白（Recombinant Human CD138 Protein），GMP级

产品货号：GMP-TL685

产品描述（Description）

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.

分子别名（Synonym）

SDC1、Syndecan-1、SYND1、SDC

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列编码人 CD138（UniProtKB/Swiss-Prot: P18827.3）表达带有 Fc 标签在 C 末端

分子量（Molecular Weight） 

重组人 CD138 蛋白 包含 463 个氨基酸，预测理论分子量为 50.8kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

以固定蛋白来增强 Saos2（人骨肉瘤细胞）对人纤维连接蛋白黏附的能力来检测，将 5×10⁴细胞/孔 加入到人 Syndocan-1 和人纤维连接蛋白（0.5ug/ml）包被板中，细胞黏附以剂量依赖性方式增强。这种作用的 ED₅₀为 5-10ug/ml

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、Syndecan-1 (CD138) Modulates Triple-Negative Breast Cancer Stem Cell Properties via Regulation of LRP-6 and IL-6-Mediated STAT3 Signaling Sherif A. Ibrahim, Hebatallah Hassan, Laura Vilardo, Sampath Katakam Kumar, Archana Vijaya Kumar, Reinhard Kelsch, Cornelia Schneider, Ludwig Kiesel, Hans Theodor Eich, Ileana Zucchi, Rolland Reinbold, Burkhard Greve, Martin Götte PLoS One. 2013; 8(12): e85737. Published online 2013 Dec 31. doi: 10.1371/journal.pone.0085737 

2、Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis Paulina M. Kowalewska, Uyen T. Nguyen, Lori L. Burrows, Alison E. Fox-Robichaud J Biomed Sci. 2016; 23: 20. Published online 2016 Feb 1. doi: 10.1186/s12929-016-0232-9

  注意：For research and manufacturing use.