Product: Anti-APRIL, clone Aprily-8
Cat. No.: MC-094 (100 μg)
Synonyms:
A-Proliferation-inducing Ligand, CD256, TNFSF
13.
Specificity:
Recognizes human APRIL and TWE-PRIL.
Species Reactivity:
Human. Does not cross-react with mouse APRIL
and TWE-PRIL. Others species not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
Recombinant human APRIL (aa 93-233)
expressed in bacteria.
Format:
100 μg of antibody at 1 mg/mL in PBS with
0.02% sodium azide. 95% purity as determined
by SDS-PAGE.
Storage:
Store at 4°C short term and at -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications:

Western blots (excellent)

Flow cytometry

Immunoprecipitation

Immunocytochemistry (excellent)

Immunohistochemistry (paraffin)
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot of total cell extracts from HEK 293
cells transfected with the indicated expression vector. Aprily-
8 reacts specifically with human APRIL.
Method: 10 μg of protein was applied to the gel. Revealed
with Aprily-8 (1 μg/mL) and HRP-coupled anti-mouse
secondary antibody.
Note: The hAPRIL construct used in this experiment is an
uncleavable fusion protein between hBAFF (aa 1-132)
and hAPRIL (aa 93-233).
Figure 2: Immunostaining of HEK 293 cells transfected with
a human APRIL expression plasmid (left panels), or mock
transfected (right panel) by Aprily-8.
Method: 3 days after transfection of cells with the indicated
constructs, cells were fixed with 4% formaldehyde 5 min at
RT. After a wash in PBS, samples were dehydrated by
washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells
were then dried and embedded in paraffin. Sections were cut,
mounted on slides and dried overnight at 50°C. Slides were
then successively washed 2x 10 min in xylol, 2x 10 min in
100% EtOH, and then treated 10 min in 100% MeOH/ 0.6%
H202 to inhibit endogenous peroxidase. Samples were
rehydrated by washes in 90%, 80%, 60% EtOH and PBS.
After micro-wave treatment, slides were washed 3x in PBS,
blocked with IgG, and incubated for 1 hour with 5μg/mL
Aprily-8 or control mouse IgG (isotype control) in 1% BSA /
1x PBS for 1 hour. After PBS washes, samples were
incubated with the secondary Ab for 1 hour, washed in PBS
and revealed with StreptABComplex/HRP (Vector) and AEC.