BRMS1,乳腺癌转移抑制基因1抗体

产品编号HZ-1015R
英文名称BRMS1
中文名称乳腺癌转移抑制基因1抗体
别 名AV003220;AW554636;Breast cancer metastasis suppressor 1 ; BRMS-1; BRMS1; BRMS 1; DKFZp564A063;MGC95128; BRMS1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
BRMS1,乳腺癌转移抑制基因1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human BRMS-1 C-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 ∑C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20∑C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ∑C.
BRMS1,乳腺癌转移抑制基因1抗体PubMedPubMed
产品介绍background:
Breast cancer metastasis-suppressor 1 (BRMS1) is 246 amino acid protein that acts as a mediator of metastasis suppression in several types of cancer including ovarian, lung, bladder, and murine mammary. BRMS1 mRNA is expressed in various tissues, including ovary, prostate, testis, and colon, but the protein is primarily detected in term placenta. BRMS1 suppresses metastasis without inhibiting tumorigenicity by modifying several metastasis-associated phenotypes. BRMS1 may participate in transcriptional regulation by binding to the mSin3/histone deacetylase complex. The expression of BRMS1 in certain cells increases connexin Cx43 expression and reduces connexin Cx32 expression. This produces a gap junction that increases intercellular communication, similar to those found in normal breast tissue. BRMS1 is stabilized by Hsp90 and may inhibit NF-ⅹB activity.

Function:
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.

Subunit:
Belongs to the BRMS1 family.

Subcellular Location:
Nucleus. Cytoplasm. Predominantly nuclear.

Tissue Specificity:
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.

BRMS1,乳腺癌转移抑制基因1抗体Post-translational modifications:
Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.

Similarity:
Belongs to the BRMS1 family.

Gene ID:
25855

Database links:
Entrez Gene: 25855 Human
Entrez Gene: 107392 Mouse
Entrez Gene: 293668 Rat
Omim: 606259 Human
SwissProt: Q9HCU9 Human
SwissProt: Q99N20 Mouse
SwissProt: Q5M7T3 Rat
Unigene: 100426 Human
Unigene: 2839 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-BRMS-1 Polyclonal Antibody, Unconjugated(HZ-1015R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lymph node tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-BRMS-1 Polyclonal Antibody, Unconjugated(HZ-1015R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining