分子量:36.6 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Cathepsin S, His Tag by the LAL method.

Buffer:50 mM MES, pH 6.5 with 100 mM NaCl

生物活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >300 pmol/min/g.

产品特性:Human Cathepsin S, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.6 kDa. The predicted N-terminus is Gln 17 (pro)  & Ser 109  (mature). The reducing (R) protein migrates as 37 kDa  (pro)  and 26 kDa (mature) in SDS-PAGE  due to glycosylation.

产品背景:Cathepsin S is also known as CTSS, which is a member of the peptidase C1 family. Cathepsin S is a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages. The catalytic activity of cathepsin S is similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.