Active人ADAM12蛋白片段-蛋白质/抗原/多肽-试剂-生物在线

产品名称： Active人ADAM12蛋白片段

英文名称： A disintegrin and metalloproteinase domain 12 ADA12_HUMAN ADAM 12 ADAM metallopeptidase domain 12 ADAM12 Disintegrin and metalloproteinase d

产品编号： ab174014

产品价格： 0

产品产地：英国

品牌商标： abcam

更新时间： null

使用范围： null

深圳市宇德立生物科技有限公司

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Our Abpromise guarantee covers the use of ab174014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

生物活性
Measured by its ability to cleave IGFBP-3. The bioactivity was measured in 100 μL reaction mixture containing 20 µg/ml of ab174014 50 mM Tris, 10 mM CaCl₂, 150 mM NaCl, pH 7.5.
应用
SDS-PAGE

Functional Studies
内毒素水平< 1.000 Eu/µg
纯度> 98 % by SDS-PAGE.
形式Lyophilised
浓度由溶解方法决定

稳定性和存储
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C long term. Avoid freeze / thaw cycle.

This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶It is recommended to reconstitute the lyophilized protein in 50 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage

别名
- A disintegrin and metalloproteinase domain 12
- ADA12_HUMAN
- ADAM 12
see all
功能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
组织特异性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
序列相似性Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
结构域The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰The precursor is cleaved by a furin endopeptidase.
细胞定位Secreted and Cell membrane.
Information by UniProt