phospho-Acinus,磷酸化腺泡Acinus蛋白抗体

产品编号HZ-3001R
英文名称phospho-Acinus (Ser1180)
中文名称磷酸化腺泡Acinus蛋白抗体
别 名Acinus (phospho S1180); Acinus (phospho Ser1180); Acinus (phospho-S1180); Acinus (phospho-Ser1180); p-Acinus (Ser1180); ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670.
规格价格0.1ml
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子
抗体来源Rabbit
phospho-Acinus,磷酸化腺泡Acinus蛋白抗体克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量148kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Acinus around the phosphorylation site of Ser1180
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-Acinus,磷酸化腺泡Acinus蛋白抗体PubMedPubMed
产品介绍background:
Chromatin condensation and nuclear fragmentation (CCNF) are the hallmarks of apoptosis. CCNF is triggered by the activation of members of the caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by Caspase 3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated Acinus L (1341 amino acids), Acinus S (583 amino acids) and Acinus S' (614 amino acids)

Function:
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

Subunit:
Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, PNN, RBM8A, RNPS1, SAP18 and ALYREF/THOC4. Forms heterodimers with RNPS1. Found in a heterotrimeric complex with ACIN1, RNPS1 and SAP18. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

Subcellular Location:
Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Note=Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

phospho-Acinus,磷酸化腺泡Acinus蛋白抗体Tissue Specificity:
Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Post-translational modifications:
Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.
Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

Similarity:
Contains 1 SAP domain.

Gene ID:
22985

Database links:
Entrez Gene: 22985 Human
Omim: 604562 Human
SwissProt: Q9UKV3 Human
Unigene: 124490 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-Acinus(Ser1180) Polyclonal Antibody, Unconjugated(bs-3001R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining