Malate dehydrogenase (MDH2) catalyzes the last reaction of the TCA or Krebs cycle, converting malate and NAD+ to oxaloacetate and NADH in the mitochondria. In reversing the reaction, MDH is also involved in gluconeogenesis, allowing oxaloacetate/malate to leave the mitochondria. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic MDH or MDH1, which is followed by conversion of oxaloacetate to phosphoenolpyruvate by phosphoenolpyruvate carboxykinase (PEPCK). The interconversion between malate and oxaloacetate also constitutes the essential steps of the malate-aspartate shuttle. This colorimetric assay is based on malate dehydrogenase-catalyzed oxidation of malate, where the resulting NADH can then convert a nearly colorless probe to a colored product; the intensity of the colored product is proportional to the amount of MDH in the sample, exhibiting maximum absorbance at 440nm.

 

Product Use

The MDH Assay kit measures the MDH activity in different types of samples, including tissue and cell lysate. This product is for research purposes only and is not for use in animals, humans, or diagnostic procedures.

 

Shipping

Shipped on dry ice.