大鼠己糖激酶1(HK1)多克隆抗体
Polyclonal Antibody to Hexokinase 1 (HK1)
[PRODUCT INFORMATION]
Immunogen: HK1
Clonality: Polyclonal
Host: Rabbit
Species Reactivity: Rat
Ig type: Rabbit IgG
Purification: Antibodies are purified by
target protein affinity chromatography.
Applications: WB, ICC, IHC-P, IHC-F
Form: Liquid
Size: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant rat HK1 (Asp413~Val540) expressed in E.coli.
Molecular Weight: 41.7kDa
Sequence: The target protein is fused with two N-terminal Tags, His-tag andGST-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVLDIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDGSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- DGSLYKMH PQYSRRFHKT LRRLVPDSDV RFLLSESGTG KGAAMVTAVAYRLAEQHRQI EETLAHFRLS KQTLMEVKKR LRTEMEMGLR KETNSKATVK MLPSFVRSIPDGTEHGDFLA LDLGGTNFRV
[ ANTIBODY SPEFICITY ]
Anti HK1 is a rabbit polyclonal antibody raised against rat HK1. This antibody hasbeen selected for its ability to recognize rat HK1 in immunohistochemical stainingand western blotting, non cross-reactive with other members of the family.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant rat HK1 (Asp413~Val540)disposed in loading buffer. 
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。