大鼠烯醇化酶(ENO3)多克隆抗体
Polyclonal Antibody to Enolase 3 (ENO3)
[PRODUCT INFORMATION]
Immunogen: MSE, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant MSE (Met3~Lys434) expressed in E.coli. 
Sequence: The target protein is fused with N-terminal His-Tag and its sequenceis listed below.
MGHHHHHHSG S-MQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELRDGDKSRYLGK GVLKAVEHIN KTLGPALLEK KLSVVDQEKV DKFMIELDGT ENKSKFGANAILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLVLPVPAF NVINGGSHAG NKLAMQEFMILPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTAIQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ISGEKLGELY KSFIKNYPVVSIEDPFDQDD WATWTSFLSG VDIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSVTESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQLMRIEEALGDK AVFAGRKFRN PKAK
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against MSE. It has beenselected for its ability to recognize MSE in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user
[IMAGES ]

[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[ QUALITY CONTROL ] 
Content:The quality control contains recombinant MSE (Met3~Lys434) disposed
in loading buffer
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。