Miz1/ZNF60,锌指蛋白60抗体

产品编号HZ-11234R
英文名称Miz1/ZNF60
中文名称锌指蛋白60抗体
别 名Miz-1; Myc-interacting zinc finger protein 1; Myc-interacting zinc finger protein; ZBT17; ZBT17_HUMAN; Zbtb17; Zinc finger and BTB domain containing protein 17; Zinc finger and BTB domain-containing protein 17; Zinc finger protein 151; Zinc finger protein 60; ZNF151; ZNF60.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞周期蛋白 转录调节因子 锌指蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
Miz1/ZNF60,锌指蛋白60抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量88kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Miz1/ZNF60
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Miz1/ZNF60,锌指蛋白60抗体PubMedPubMed
产品介绍background:
The Myc family, including c-Myc-, N-Myc- and L-Myc, are nuclear proteins with relatively short half lives that contribute an important role in cellular processes such as proliferation, differentiation, apoptosis and transformation. The c-Myc protein activates transcription as part of a heteromeric complex with a number of interacting partners, including Max and Mxi 1; however the transforming properties of the Myc proto-oncogene are believed to be associated with Myc-mediated transcriptional repression. A POZ domain Zn finger protein, designated Miz-1 for Myc-interacting Zn finger protein-1, is a specific target of Myc-induced gene repression. Miz-1 interacts with Myc, but not Max or other Myc partners, and binding of Myc to Miz-1 requires the helix-loop-helix domain of Myc and a short amphipathic helix located in the carboxy-terminus of Miz-1. Miz-1 associates with DNA elements on the adenovirus major late and cyclin D1 promoters and activates transcription of both promoters. Expression of Miz-1 induces potent growth arrest function, and this latency is reversed by the addition of Myc.

Function:
May function as a housekeeping DNA-binding protein that regulates the expression of specific genes. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Also has potent growth arrest activity, probably through inhibition of cell cycle progression. Required for early embryonic development during gastrulation.

Miz1/ZNF60,锌指蛋白60抗体Subunit:
Homooligomerizes (via the BTB/POZ domain), multimerization is required for DNA binding. Interacts (via the C-terminal zinc fingers) with GIF1; the interaction results in the recruitment of MYB to the CDKN1A/p21 and CDKN1B promoters and repression of transcription. Interacts with TRAF2, interfering with the binding of UBC13 to TRAF2, and inhibiting TRAF2 E3 ligase activity (By similarity). Interacts with MYC (via the C-terminal helix-loop-helix motif): the interaction inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 1 BTB (POZ) domain.
Contains 13 C2H2-type zinc fingers.

Database links:
UniProtKB/Swiss-Prot: Q13105.3

Miz1/ZNF60,锌指蛋白60抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.