CACNB3,L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体

产品编号HZ-11982R
英文名称CACNB3
中文名称L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体
别 名CAB3; CACB3_HUMAN; Cacnb3; CACNLB3; Calcium Channel Voltage Dependent Beta 3 Subunit; Calcium channel voltage-dependent subunit beta 3; FLJ58949; Voltage-dependent L-type calcium channel subunit beta-3.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Sheep, hu, mo, rat, dog, cow, shp, hrs, pig, Rb
CACNB3,L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体产品应用IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CACNB3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
CACNB3,L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体PubMedPubMed
产品介绍background:
Voltage-dependent calcium channels are essential for the release of neurotransmitters. L-type (long lasting current) voltage-dependent calcium channels are composed of four subunits: an Alpha1 subunit, a Beta subunit, a Beta subunit and an Alpha2 Gamma subunit. The Beta subunit is encoded by four genes, designated Beta1-Beta4, all of which contribute to the diversity of calcium currents and are involved in membrane trafficking of the Beta subunit. L-type Ca++ CP Beta3, also known as CACNB3 (Calcium channel voltage-dependent subunit beta 3), CACNLB3 or CAB3, is a 484 amino acid protein that contains one SH3 domain and is expressed in ovary, brain and smooth muscle. Functioning as one of the four components of the Beta subunit, L-type Ca++ CP Beta 3 increases the peak calcium current in voltage-dependent calcium channels, thereby shifting the voltage dependencies of activation and inactivation and controlling G protein inhibition and Beta membrane targeting. Two isoforms of L-type Ca++ CP Beta3 exist due to alternative splicing events.

Function:
The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.

CACNB3,L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体Subunit:
The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. Interacts with CACNA2D4. Interacts with FASLG.

Tissue Specificity:
Expressed mostly in brain, smooth muscle and ovary.

Similarity:
Belongs to the calcium channel beta subunit family.
Contains 1 SH3 domain.

Gene ID:
784

Database links:
Entrez Gene: 784 Human
Entrez Gene: 12297 Mouse
Entrez Gene: 25297 Rat
Omim: 601958 Human
SwissProt: P54284 Human
SwissProt: P54285 Mouse
SwissProt: P54287 Rat
Unigene: 250712 Human
Unigene: 3544 Mouse
Unigene: 2808 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CACNB3 Polyclonal Antibody, Unconjugated(HZ-11982R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining