• 靶标种属Mouse
• 经测试应用IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details
• 偶联物Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
• 存储溶液Preservative: 0.02% Sodium azide
  Constituents: PBS, 30% Glycerol, 1% BSA
• 浓度200 µg 浓度为 2 mg/ml 500 µg 浓度为 2 mg/ml 
  
• 纯度Immunogen affinity purified
• 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
• 克隆多克隆
• 同种型IgG
• 常规说明

  The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Mouse
   
  • IgG
   
  • Fluorophore
   
  • Alexa Fluor® 488

应用

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) 图像

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  IHC - Wholemount - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)This image is courtesy of an anonymous Abreview.

  IHC - Wholemount of Caenorhabditis elegans embryo labelling RNA polymerase II CTD repeat YSPTSPS withab817. Sample was incubated with primary antibody (1/100 in PBS + 3% BSA + 0.1% Triton-X 100) for 24 hours at 4°C. ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

  See Abreview

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150113 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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  Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488) (ab150113)

  Overlay histogram showing HeLa cells stained with ab26(red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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  Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)