信号素4D(SEMA4D)重组蛋白

YB430Mu013
Recombinant Semaphorin 4D (SEMA4D)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
12th Edition (Revised in Aug, 2016)
[ PROPERTIES ]
信号素4D(SEMA4D)重组蛋白Source: Prokaryotic expression. Host: E. coli
Residues: Phe502~Phe636
Tags: N-terminal His-Tag
Tissue Specificity: Brain Intestine. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 16.2kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
信号素4D(SEMA4D)重组蛋白Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
信号素4D(SEMA4D)重组蛋白observed. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE