• 特异性ab195712 endogenous levels of Caspase-3 p17 only when phosphorylated at Serine 150.
• 经测试应用WBmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide within Human Caspase-3 p17 (phospho S150). The exact sequence is proprietary. A synthesized peptide derived from human Caspase-3 p17 around the phosphorylation site of Serine 150
  Database link: P42574

• 阳性对照
  • Etoposide treated Jurkat whole cell lysate.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
• 浓度100 µg 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Cell Biology
   
  • Apoptosis
   
  • Intracellular
   
  • Caspases etc
   
  • Caspases

  • Cancer
   
  • Invasion/microenvironment
   
  • Apoptosis
   
  • Caspases

  • Cell Biology
   
  • Proteolysis / Ubiquitin
   
  • Proteolytic enzymes
   
  • Other proteases

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolism processes
   
  • Apoptosis

  • Cancer
   
  • Cell Death
   
  • Apoptosis
   
  • Apoptotic Markers
   
  • Caspases

  • Cancer
   
  • Cell Death
   
  • Apoptosis
   
  • Metabolism

应用

靶标

• 功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
  -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
• 组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
• 序列相似性Belongs to the peptidase C14A family.
• 翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
  S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
• 细胞定位Cytoplasm.

• Anti-Caspase-3 (phospho S150) antibody 图像

  • 

    Western blot - Anti-Caspase-3 p17 (phospho S150) antibody (ab195712)
    All lanes : Anti-Caspase-3 (phospho S150) antibody (ab195712) at 1/500 dilution
    
    Lane 1 : Etoposide treated Jurkat whole cell lysate with antigen-specific peptide
    Lane 2 : Etoposide treated Jurkat whole cell lysate
    
    
    Predicted band size : 32 kDa