ARFGAP1 polyclonal antibody
产品名称： ARFGAP1 polyclonal antibody
英文名称： ARFGAP1 polyclonal antibody
产品编号： PAB4255
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ARFGAP1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ARFGAP1.
- Host:
- Rabbit
- Reactivity:
- Human, Rat
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Small-molecule synergist of the Wnt/beta-catenin signaling pathway.
Zhang Q, Major MB, Takanashi S, Camp ND, Nishiya N, Peters EC, Ginsberg MH, Jian X, Randazzo PA, Schultz PG, Moon RT, Ding S.Proc Natl Acad Sci U S A. 2007 May 1;104(18):7444-8. Epub 2007 Apr 25.
- Applications
- Western Blot (Recombinant protein)
- Western blot analysis of ARFGAP1 polyclonal antibody (Cat # PAB4255) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right) . ARFGAP1 (arrow) was detected using the purified ARFGAP1 polyclonal antibody (Cat # PAB4255) . Data courtesy of Dr . Dan Cassel, Department of Biology, Technion, Haifa, Israel .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARFGAP1 polyclonal antibody (Cat # PAB4255) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Entrez GeneID:
- 55738
- Protein Accession#:
- NP_060679;Q8N6T3
- Gene Name:
- ARFGAP1
- Gene Alias:
- ARF1GAP,HRIHFB2281,MGC39924
- Gene Description:
- ADP-ribosylation factor GTPase activating protein 1
- Omim ID:
- 608377
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- ADP-ribosylation factor 1 GTPase activating protein,ARF1-directed GTPase-activating protein,GAP protein,OTTHUMP00000031551
- Gene Pathway