Rho关联含卷曲螺旋结合蛋白激酶1(Rock1)重组蛋白

YB705Mu016
Recombinant Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 Rho关联含卷曲螺旋结合蛋白激酶1(Rock1)重组蛋白[ PROPERTIES ]
Residues: Leu1094~Gly1323 (Accession # P70335),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cytoplasm, cytoskeleton,
microtubule organizing center, centrosome,
centriole. Golgi apparatus membrane; Peripheral
membrane protein. Cell projection, bleb. Cell
membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
 Rho关联含卷曲螺旋结合蛋白激酶1(Rock1)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.3
Predicted Molecular Mass: 29.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 Rho关联含卷曲螺旋结合蛋白激酶1(Rock1)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS - LLDLSDS TSVASFPSAD
ETDGNLPESR IEGWLSVPNR GNIKRYGWKK QYVVVSSKKI LFYNDEQDKE QSSPSMVLDI
DKLFHVRPVT QGDVYRAETE EIPKIFQILY ANEGECRKDI EVEPVQQGEK TNFQNHKGHE
FIPTLYHFPA NCEACAKPLW HVFKPPPALE CRRCHVKCHR DHLDKKEDLI SPCKVSYDVT
SARDMLLLAC SQDEQKKWVT HLVKKIPKNP PSG