外皮蛋白(EVPL)重组蛋白

YB549Hu013
Recombinant Envoplakin (EVPL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
外皮蛋白(EVPL)重组蛋白[ PROPERTIES ]
Residues: Lys3~Met258
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q92817
Host: E. coli
Subcellular Location: Cell junction. Cytoplasm. Cytoskeleton.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
外皮蛋白(EVPL)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 58.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
外皮蛋白(EVPL)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
KGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA DQVERDILET
QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD VDKARRLKHP QAEEIEKDIK
QL HE RVTQE C AEY RA LYE KM VLPP DVGP RV DWA RV LE QKQ KQV CAG QYGP
GMAELEQQIA EHNILQKEID AYGQQLRSLV GPDAATIRSQ YRDLLKAASW RGQSLGSLYT
HLQGCTRQLS ALAEQQRRIL QQDWSDLM