分子量:95 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human EGF R, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human EGF, Tag Free (Catalog # EGF-H4114) at 10 μg /mL (100 μL/well ) can bind Human EGF R, Fc Tag (Catalog # EGR-H5252) with a linear range of 0.025-0.5 μg /mL. Measured by its ability to bind Human EGF, Tag Free (Cat# EGF-H4114) in the SPR assay (Biacore 2000) with an estimated KD < 1nM.
Validated CetuxiMab binding in a functional ELISA.

产品特性:Human EGF R, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 95 kDa. The predicted N-terminus is Leu 25. The reducing (R) protein migrates as 130-140 kDa in SDS-PAGE  due to glycosylation.

产品背景:The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.