分子量:35.8 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CD55, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:MeasuredbyitsbindingabilityinafunctionalELISA.  When Human CD55, His Tag is coated at 1 μg/mL (100 μl/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15-2.0 μg/mL.

产品特性:Human CD55, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35 . The reducing (R) protein migrates as 50-65 kDa in SDS-PAGE  due to glycosylation.

产品背景:CD55 is alsoknownasDAFordecayacceleratingfactor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologicallygenerated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C’ attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.