ZNF828,锌指蛋白828抗体

产品编号HZ-9607R
英文名称ZNF828
中文名称锌指蛋白828抗体
别 名chromosome 13 open reading frame 8; zinc finger protein 828; FLJ90413; KIAA1802; Zinc finger protein KIAA1802; CHAP1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 转录调节因子 锌指蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
ZNF828,锌指蛋白828抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量89kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ZNF828/C13orf8
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ZNF828,锌指蛋白828抗体PubMedPubMed
产品介绍background:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF828, is a 812 amino acid protein that contains one C2H2-type zinc finger and is localized to the cytoplasm and the nucleus. The gene encoding ZNF828 maps to chromosome 13. Comprising nearly 4% of the human genome, chromosome 13 contains around 114 million base pairs and encodes over 400 genes. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.

Function:
Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.

Subunit:
Interacts with MAD2L2. Interacts with POGZ, CBX1, CBX3 and CBX5.

Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.

Post-translational modifications:
Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.

Similarity:
Contains 1 C2H2-type zinc finger.

Gene ID:
283489

ZNF828,锌指蛋白828抗体Database links:
Entrez Gene: 283489 Human
Entrez Gene: 101994 Mouse
Entrez Gene: 306647 Rat
SwissProt: Q96JM3 Human
SwissProt: Q8K327 Mouse
Unigene: 7542 Human
Unigene: 289346 Mouse
Unigene: 412576 Mouse
Unigene: 3851 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (HZ-9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF828) Polyclonal Antibody, Unconjugated (HZ-9607R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.