SIRT2 monoclonal antibody (M01), clone 4B11
产品名称： SIRT2 monoclonal antibody (M01), clone 4B11
英文名称： SIRT2 monoclonal antibody (M01), clone 4B11
产品编号： H00022933-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant SIRT2.
- Immunogen:
- SIRT2 (AAH03012, 128 a.a. ~ 227 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- YTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSL
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of SIRT2 expression in transfected 293T cell line by SIRT2 monoclonal antibody (M01), clone 4B11.
Lane 1: SIRT2 transfected lysate(39.5 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to SIRT2 on HeLa cell . [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged SIRT2 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of SIRT2 over-expressed 293 cell line, cotransfected with SIRT2 Validated Chimera RNAi ( Cat # H00022933-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIRT2 monoclonal antibody (M01), clone 4B11 (Cat # H00022933-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 22933
- GeneBank Accession#:
- BC003012
- Protein Accession#:
- AAH03012
- Gene Name:
- SIRT2
- Gene Alias:
- SIR2,SIR2L,SIR2L2
- Gene Description:
- sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
- Omim ID:
- 604480
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene. [provided by RefSeq
- Other Designations:
- silencing information regulator 2-like,silent information regulator 2,sir2-related protein type 2,sirtuin 2,sirtuin type 2