甘氨酸受体α2(GLRα2)重组蛋白

YB857Ra012
Recombinant Glycine Receptor Alpha 2 (GLRa2)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
甘氨酸受体α2(GLRα2)重组蛋白[ PROPERTIES ]
Residues: Lys28~Gln253
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P22771
Host: E. coli
Subcellular Location: Cell junction, synapse,
postsynaptic cell membrane. Multi-pass membrane
protein. Cell membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
甘氨酸受体α2(GLRα2)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 29.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
甘氨酸受体α2(GLRα2)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
KDH DSRSGKHPSQ TLSPSDFLDK LMGRTSGYDA RIRPNFKGPP VNVTCNIFIN
SFGSVTETTM DYRVNIFLRQ QWNDSRLAYS EYPDDSLDLD PSMLDSIWKP DLFFANEKGA
NFHDVTTDNK LLRISKNGKV LYSIRLTLTL SCPMDLKNFP MDVQTCTMQL ESFEYTMNDL
IFEWLSDGPV QVAEGLTLPQ FILKEEKELG YCTKHYNTGK FTCIEVKFHL ERQ