FITC标记的微小染色体维持缺陷蛋白5抗体
产品名称： FITC标记的微小染色体维持缺陷蛋白5抗体
英文名称： Anti-MCM5/FITC
产品编号： HZ-2721R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-MCM5/FITC Conjugated antibody
FITC标记的微小染色体维持缺陷蛋白5抗体
| 英文名称 | Anti-MCM5/FITC |
| 中文名称 | FITC标记的微小染色体维持缺陷蛋白5抗体 |
| 别 名 | CDC 46; CDC46; CDC46 homolog; Cell division cycle 46; DNA replication licensing factor; DNA replication licensing factor MCM 5; DNA replication licensing factor MCM5; MCM 5; MGC5315; Minichromosome maintenance deficient (S. cerevisiae) 5; Minichromosome maintenance deficient 5; Minichromosome maintenance deficient protein 5; P1 CDC46; MCM5_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 发育生物学 细胞凋亡 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 82kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCM5 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-induced Stat1 Alpha transcription activation. Function: MCM5 (Mini Chromosome Maintainance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy. Subcellular Location: Nucleus. Similarity: Belongs to the MCM family. Contains 1 MCM domain. Database links: Entrez Gene: 4174 Human Entrez Gene: 17218 Mouse Omim: 602696 Human SwissProt: P33992 Human SwissProt: P49718 Mouse Unigene: 517582 Human Unigene: 5048 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
包括McM2、MCM3、MCM4（CDC21）、MCM5（CDC46）、MCM6（MIS5）和MCM7（CDC47）的蛋白质的微染色体维持（MCM）家族是DNA复制的调节器，其作用是确保复制仅在细胞周期中发生一次。MCM蛋白在细胞生长过程中表达增加，在G1期至S期达到高峰。MCM蛋白均含有ATP结合基序，其预测介导双链DNA的ATP依赖性开放。MCM蛋白受E2F转录因子调节，其诱导MCM表达，并通过与MCM蛋白相互作用的蛋白激酶维持细胞的后复制状态。MCM2/MCM4复合物作为CDC2/cyclin B的底物发挥作用。McM3的裂解，可由胱天蛋白酶抑制剂阻止，导致MCM复合物凋亡过程中的失活，MCM复合物至少由McM2 - 6组成。MCM4、MCM6和MCM7组成的复合物参与了DNA解旋酶的活性，MCM5参与了IFN诱导的STAT1α转录激活。