血小板活化因子抗体
产品名称: 血小板活化因子抗体
英文名称: PAF
产品编号: hz-1119R
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Rabbit Anti-PAF antibody
产品编号 | hz-1119R |
英文名称 | PAF |
中文名称 | 血小板活化因子抗体 |
别 名 | platelet-activating factor; 1-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine; 1-O-Palmityl-2-acetyl-sn-glycero-3-phosphocholine monohydrate; 2-Acetyl-1-hexadecyl-sn-glycero-3-phosphocholine; 3-sn-Phosphatidylcholine; 1-hexadecyl-2-acetyl, PAF(C16. |
产品类型 | 药物与化合物抗体 |
研究领域 | 药物及化合物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat, PAF |
产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 0.524kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated PAF: |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Platelet-activating factor, a compound that reduces inflammation by increasing permeability of blood vessels and contracting various involuntary muscles such as those in airways. It is a phospholipid with a wide range of proinflammatory actions, is immediately degraded and inactivated in vivo by PAF acetylhydrolase. SWISS: N/A CAS: 74389-68-7 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 血小板活化因子(platelet-activating factor,PAF)是一种内源性具有生物活性的磷脂。由中性粒细胞、血小板、肥大细胞、内皮细胞和巨噬细胞产生。除早期发现它有强烈引起血小板聚集和脱颗粒作用外,近年来的研究发现它还具有广泛的生物活性,作为一种独特的细胞因子参与体内多种生理、病理过程,包括激活中性粒细胞,使其聚集、趋化、释放氧自由基和白三烯,抑制T细胞增殖,刺激B细胞合成IgG和IgE,抑制心肌收缩力,增加血管通透性,引起体循环血压下降和肺动脉高压等。由于它具有广泛的高强度的生物活性,故PAF被认为是与过敏反应有关的关键性介质之一。 |
产品图片 |
Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min Incubation: Anti-PAF Polyclonal Antibody, Unconjugated(bs-1119R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |