ERF,转录因子ERF抗体

产品编号HZ-13096R
英文名称ERF
中文名称转录因子ERF抗体
别 名ERF; ERF_HUMAN; ETS domain containing transcription factor ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2; PE2.
说 明 书0.1ml 0.2ml
研究领域细胞生物 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
ERF,转录因子ERF抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量59kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ERF/Ets2 Repressor Factor
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ERF,转录因子ERF抗体PubMedPubMed
产品介绍background:
ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.

Function:
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.

ERF,转录因子ERF抗体Subcellular Location:
Nucleus.

Tissue Specificity:
Highest levels in testis, ovary, pancreas, and heart.

Post-translational modifications:
Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526.
Phosphorylation regulates the activity of ERF.

Similarity:
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.

Gene ID:
2077

Database links:
Entrez Gene: 2077 Human
Entrez Gene: 13875 Mouse
Entrez Gene: 292721 Rat
Omim: 611888 Human
SwissProt: P50548 Human
SwissProt: P70459 Mouse
Unigene: 655969 Human
Unigene: 8068 Mouse
Unigene: 9093 Rat

ERF,转录因子ERF抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.