ACO2 polyclonal antibody
产品名称： ACO2 polyclonal antibody
英文名称： ACO2 polyclonal antibody
产品编号： PAB3750
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ACO2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ACO2.
- Host:
- Rabbit
- Reactivity:
- Human, Rat
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Modulation of mitochondrial aconitase on the bioenergy of human prostate carcinoma cells.
Juang HH.Mol Genet Metab. 2004 Mar;81(3):244-52.
- 2.
- Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism.
Bota DA, Davies KJ.Nat Cell Biol. 2002 Sep;4(9):674-80.
- 3.
- The aconitase family: three structural variations on a common theme.
Gruer MJ, Artymiuk PJ, Guest JR.Trends Biochem Sci. 1997 Jan;22(1):3-6.
- Applications
- Western Blot (Tissue lysate)
- Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent (Pierce), containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma) ; PVDF membrane was incubated in ACO2 polyclonal antibody (Cat # PAB3750). Solution : 1 : 1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 °C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
- Entrez GeneID:
- 50
- Protein Accession#:
- NP_001089;Q99798
- Gene Name:
- ACO2
- Gene Alias:
- ACONM,MGC20605,MGC33908
- Gene Description:
- aconitase 2, mitochondrial
- Omim ID:
- 100850
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq
- Other Designations:
- OTTHUMP00000042146,OTTHUMP00000165920,aconitase 2,aconitate hydratase,citrate hydro-lyase
- Gene Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glyoxylate and dicarboxylate metabolism
- Metabolic pathways
- Reductive carboxylate cycle (CO2 fixation)