PRMT7 polyclonal antibody
产品名称： PRMT7 polyclonal antibody
英文名称： PRMT7 polyclonal antibody
产品编号： PAB2180
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PRMT7.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of humanPRMT7.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.
Lee JH, Cook JR, Yang ZH, Mirochnitchenko O, Gunderson SI, Felix AM, Herth N, Hoffmann R, Pestka S.J Biol Chem. 2005 Feb 4;280(5):3656-64. Epub 2004 Oct 19.
- 2.
- PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity.
Miranda TB, Miranda M, Frankel A, Clarke S.J Biol Chem. 2004 May 28;279(22):22902-7. Epub 2004 Mar 24.
- 3.
- The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Frankel A, Yadav N, Lee J, Branscombe TL, Clarke S, Bedford MT.J Biol Chem. 2002 Feb 1;277(5):3537-43. Epub 2001 Nov 27.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of PRMT7 (arrow) using PRMT7 polyclonal antibody (Cat # PAB2180).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 54496
- Protein Accession#:
- NP_061896
- Gene Name:
- PRMT7
- Gene Alias:
- FLJ10640,KIAA1933
- Gene Description:
- protein arginine methyltransferase 7
- Omim ID:
- 610087
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM
- Other Designations:
- protein arginine N-methyltransferase 7