ACSL1抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
ACSL1抗体

ACSL1抗体

商家询价

产品名称: ACSL1抗体

英文名称: ACSL1Antibody

产品编号: XY13989-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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ACSL1抗体

Applications
In-house applications ELISA, WB, IHC, IP, IF     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 6 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse, rat
Images
Prev12NextView more validation data
  • 13989-1-AP;HepG2 cellsHepG2 cells were subjected to SDS PAGE followed by western blot with 13989-1-AP(ACSL1 antibody) at dilution of 1:800
  • 13989-1-AP;mouse liver tissueIP Result of anti-ACSL1 (IP:13989-1-AP, 4ug; Detection:13989-1-AP 1:600) with mouse liver tissue lysate 4000ug.
  • 13989-1-AP;human liver cancer tissueImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13989-1-AP( ACSL1 Antibody) at dilution of 1:50 (under 10x lens)
  • 13989-1-AP;human liver cancer tissueImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13989-1-AP( ACSL1 Antibody) at dilution of 1:50 (under 40x lens)
  • 13989-1-AP;HepG2 cellsImmunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13989-1-AP( ACSL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in HepG2 cells, human cerebellum tissue, rat liver tissue
Positive IP detected in Mouse liver tissue
Positive IHC detected in Human liver cancer tissue, human heart tissue
Positive IF detected in HepG2 cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
ACSL1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen ACSL1 fusion protein ag5059 Full name Acyl-CoA synthetase long-chain family member 1
Calculated molecular
weight
78 kDa Observed molecular 
weight
68 kDa,78 kDa
GenBank accession 
number
BC050073 Gene ID (NCBI) 2180
Gene symbol ACSL1 Synonyms ACS1, ACSL1, Acyl CoA synthetase 1, FACL1, FACL2, LACS, LACS 1, LACS 2, LACS1, LACS2, Palmitoyl CoA ligase 1, Palmitoyl CoA ligase 2
BackgroundACSL1抗体

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.ACSL1抗体

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