HES4,转录因子HES4抗体

产品编号HZ-11567R
英文名称HES4
中文名称转录因子HES4抗体
别 名bHLH factor Hes4; Hairy and enhancer of split 4; Transcription factor HES 4; HES4_HUMAN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Sheep,
HES4,转录因子HES4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量24kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human HES4 (1-100aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
HES4,转录因子HES4抗体PubMedPubMed
产品介绍background:
The Drosophila hairy and Enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila hairy and Enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

Function:
HES4 may be a transcriptional repressor. It is expressed in stomach, pancreas, ovary, uterus, eye and bone. It has an N-terminal bHLH domain.

Subunit:
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family

Subcellular Location:
Nuclear

Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 Orange domain.

HES4,转录因子HES4抗体Database links:
UniProtKB/Swiss-Prot: Q9HCC6.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.