phospho-Caspase 6 (Ser257),磷酸化半胱胺酸蛋白酶蛋白6抗体

产品编号HZ-5240R
英文名称phospho-Caspase 6 (Ser257)
中文名称磷酸化半胱胺酸蛋白酶蛋白6抗体
别 名Apoptosis-related cysteine protein; Apoptosis Related Cysteine Protease; Apoptotic cysteine protease MCH2; Apoptotic protease MCH 2; Apoptotic protease MCH2; Casp 6; Casp6; Caspase 6 apoptosis related cysteine protease; Caspase 6 precursor; Caspase6; Human cysteine protease Mch2 isoform alpha; Mch 2; Mch2; CASP6_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 免疫学 神经生物学 信号转导 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
phospho-Caspase 6 (Ser257),磷酸化半胱胺酸蛋白酶蛋白6抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量11kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Caspase 6 around the phosphorylation site of Ser257
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-Caspase 6 (Ser257),磷酸化半胱胺酸蛋白酶蛋白6抗体PubMedPubMed
产品介绍background:
Caspase-6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Subunit is compose of heterodimer of a18 kDa (p18) and a 11 kDa (p11) subunit. Subcellular location at cytoplasmic. Cleavages by CPP32, caspase-8 or -10 generate the two active subunits. It belongs to the peptidase C14 family.

Function:
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

Similarity:
Belongs to the peptidase C14A family.

phospho-Caspase 6 (Ser257),磷酸化半胱胺酸蛋白酶蛋白6抗体Database links:
UniProtKB/Swiss-Prot: P55212.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Caspase-6 与Caspase-3有38%的同源性，属胱氨酸-天冬氨酸蛋白酶家族。在凋亡执行阶段起中心作用，可被Caspase-7、-8、-10剪切。它是唯一一个可以剪切核纤层蛋白的Caspase.

产品图片

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-Caspase-6(Ser257) Polyclonal Antibody, Unconjugated(HZ-5240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining