CstF-64,细胞分裂相关蛋白CSTF64抗体

产品编号HZ-6995R
英文名称CstF-64
中文名称细胞分裂相关蛋白CSTF64抗体
别 名CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 64 kDa subunit, tau variant; CSTF 64 kDa subunit, tau variant; KIAA0689; TauCstF64; CSTFT_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep,
CstF-64,细胞分裂相关蛋白CSTF64抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量64kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CSTF2T/CstF-64 (51-150aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CstF-64,细胞分裂相关蛋白CSTF64抗体PubMedPubMed
产品介绍background:
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

Function:
May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs

Subcellular Location:
Nuclear

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

CstF-64,细胞分裂相关蛋白CSTF64抗体Similarity:
Contains 1 RRM (RNA recognition motif) domain.

Database links:
UniProtKB/Swiss-Prot: Q9H0L4.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.