phospho-Cortactin (Tyr421),磷酸化皮层肌动蛋白抗体

产品编号HZ-14000R
英文名称phospho-Cortactin (Tyr421)
中文名称磷酸化皮层肌动蛋白抗体
别 名Cortactin (phospho Y421); p-Cortactin (phospho Y421); Amplaxin; CTTN; EMS 1; EMS1; FLJ34459; Mammary tumor and squamous cell carcinoma associated; Oncogene EMS1; p80/85 src substrate; Src substrate cortactin; SRC8_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Cow, Horse, Rabbit,
phospho-Cortactin (Tyr421),磷酸化皮层肌动蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量62kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Cortactin around the phosphorylation site of Tyr421
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-Cortactin (Tyr421),磷酸化皮层肌动蛋白抗体PubMedPubMed
产品介绍background:
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Function:
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.

Subcellular Location:
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.

Post-translational modifications:
Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

Similarity:
Contains 7 cortactin repeats.
Contains 1 SH3 domain.

Gene ID:
2017

Database links:
Entrez Gene: 2017 Human
Entrez Gene: 13043 Mouse
Entrez Gene: 60465 Rat
GenBank: NM_138565 Human
GenBank: NM_007803 Mouse
Omim: 164765 Human
SwissProt: Q14247 Human
SwissProt: Q60598 Mouse
Unigene: 596164 Human
Unigene: 205601 Mouse
Unigene: 107869 Rat

phospho-Cortactin (Tyr421),磷酸化皮层肌动蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.