ADAM12抗体
Applications
| In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, IP, WB |
Publications |
12 citations –view here |
| Species specificity |
Human, Mouse; other species not tested. |
| Published Species |
Human, mouse |
|
Images
Prev12Next
 mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP(ADAM12 antibody) at dilution of 1:300 IP Result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug. Immunohistochemical of paraffin-embedded human endometrial cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:100 (under 10x lens) Immunohistochemical of paraffin-embedded human endometrial cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:100 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using ADAM12 antibody 14139-1-AP at 1:25 and Rhodamine-labeled goat anti-rabbit IgG (red).
|
| Positive WB detected in |
Mouse brain tissue, HeLa cells, human placenta tissue, mouse colon tissue, mouse heart tissue, Y79 cells |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human endometrial cancer tissue, human breast cancer tissue, human placenta tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100 |
ADAM12抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
ADAM12 fusion protein ag5206 |
Full name |
ADAM metallopeptidase domain 12 |
Calculated molecular
weight |
100 kDa |
Observed molecular
weight |
97kd,68kd |
GenBank accession
number |
BC060804 |
Gene ID (NCBI) |
8038 |
| Gene symbol |
ADAM12 |
Synonyms |
ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA |
Background ADAM12抗体
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ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12’s ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein’s role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. This antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90kd mature(processed form that lack the prodomain);50-68kd degradation product and 27kd prodomain. ADAM12抗体
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