分子量:82 kDa

纯度:>88% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human COMP, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when Human COMP, His Tag immobilized at 1 g/well. More than 45% of ATDC-5 cell adhesion will be induced.

产品特性:Human COMP, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 82 kDa. The predicted N-terminus is Gln 21. The reducing (R) protein migrates as 110-130 kDa in SDS-PAGE  due to glycosylation.

产品背景:Cartilage oligomeric matrix protein (COMP) is also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contains 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.