2X TaqMan Fast qPCR 预混液

附带条件

特技

2xTaqman快速qPCR主混合物是一种随时可用的鸡尾酒，含有快速热启动Taq DNA聚合酶、MgSO4、DNTP混合物和一些稳定剂。它提供了一个2X主混合物包含所有成分，除了引物，探针和模板快速循环探针实时PCR。快速热启动Taq DNA聚合酶和专有缓冲系统减少了引物二聚体的形成，增强了实时qPCR扩增特异性和高检测灵敏度。DNF缓冲液对存在多个非特异性扩增的引物是特异性的。对于一些DNA序列没有良好的引物，DNF缓冲液可以按照以下方案加入到反应混合物中。ROX参考染料不包括在TaqMan快速qPCR主混合中，适用于不需要通过ROX参考标准化的实时PCR仪器，如转子基因？DNA引擎OpTICON？Opticon？2，铬4？实时探测器，Mastercycler？EP RealPLEX，智能循环器？Roche LightCycler？480，Roche LightCycler？纳米，生物RAD CFX96，和Illumina ECO？.

特技

2×TaqMan快速qPCR大师混合物是一种现成的鸡尾酒，它只添加引物探针和模板来建立反应混合物。

热启动Taq DNA聚合酶的独特热启动方式和最佳缓冲体系确保了扩增的特异性和高效性。

在2×TaqMan快速qPCR主混合中，热启动Taq DNA聚合酶具有比普通热启动Taq DNA聚合酶更快的延伸率，使得实时qPCR的总反应时间更短。

独特的DNF缓冲液对存在多个非特异性扩增的引物具有特异性。

共有三种2×SG快速qPCR主混合物，包括NO /低/高ROX参考，适用于所有的实时PCR仪器。

船首

一个词

特赦

2XTAQMAN快速qPCR主混合物；DNF缓冲液；灭菌DDH2O。

概述

The 2xTaqMan Fast qPCR Master Mix is a ready-to-use cocktail containing fast hot start Taq DNA Polymerase, MgSO4, dNTP Mix and some stabilizers. It is supplied as a 2X Master Mix containing all components except primers, probe and template for fast-cycling probe-based real-time PCR. The fast hot start Taq DNA Polymerase and proprietary buffer system reduce the formation of the primer dimers, enhance the amplification specificity and high detection sensitivity in real-time qPCR. The DNF Buffer is specific for the primers which exist multi nonspecific amplification. For some DNA sequences there are no good primers, the DNF Buffer can be added to the reaction mix according to the following protocol. The ROX reference dye is not included in the TaqMan Fast qPCR Master Mix and applicable to the real time PCR instruments that are no need to normalization by the Rox reference, like Rotor-Gene?, DNA Engine Opticon?, Opticon? 2, Chromo 4? Real-Time Detector, Mastercycler? ep realplex, Smart Cycler?, Roche LightCycler? 480, Roche LightCycler? Nano, Bio-Rad CFX96, and Illumina Eco?.

特点

• The 2×TaqMan Fast qPCR Master Mix is a ready-to-use cocktail, which only add primers probe and template to set up the reaction mix.
• The unique hot-start way of the hot start Taq DNA Polymerase and optimal buffer system ensure the high amplification specificity and efficiency.
• The hot start Taq DNA Polymerase in the 2×TaqMan Fast qPCR Master Mix has faster extension rates than common hot start Taq DNA Polymerase，making the total reaction time of the real time qPCR shorter.
• The unique DNF Buffer is specific for the primers which exist multi nonspecific amplification.
• There are three kinds of 2×SG Fast qPCR Master Mix including no/low/high Rox reference，applying to all the the real time PCR instruments.

应用

基因表达分析、拷贝数分析

组份

2xTaqMan Fast qPCR Master Mix; DNF Buffer; Sterilized ddH2O.