DCPS,清道夫脱帽酶/热休克蛋白1抗体

产品编号HZ-12987R
英文名称DCPS
中文名称清道夫脱帽酶/热休克蛋白1抗体
别 名1700001E16Rik; AA408441; DCPS; DCPS_HUMAN; DCS 1; DCS-1; DCS1; Decapping enzyme scavenger; heat shock-like protein 1; Hint 5; Hint related 7meGMP directed hydrolase; HINT-5; Hint-related 7meGMP-directed hydrolase; HINT5; Histidine triad protein member 5; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; HSL1; HSPC015; m7GpppX diphosphatase; MGC124934; mRNA decapping enzyme; OTTMUSP00000042220; Scavenger mRNA decapping enzyme DcpS; Scavenger mRNA-decapping enzyme DcpS.
说 明 书0.1ml 0.2ml
研究领域细胞生物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
DCPS,清道夫脱帽酶/热休克蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DCPS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DCPS,清道夫脱帽酶/热休克蛋白1抗体PubMedPubMed
产品介绍background:
Eukaryotic cells primarily utilize exoribonucleases and decapping enzymes to degrade their mRNA. DcpS is a scavenger pyrophosphatase that hydrolyzes the residual cap structure following 3' to 5' decay of an mRNA. Following mRNA degradation DcpS releases N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. The central histidine within the DcpS HIT motif is critical for decapping activity and defines the HIT motif as a new mRNA decapping domain, making DcpS the first member of the HIT family of proteins with a defined biological function. HIT proteins are homodimeric and contain two conserved 100-amino-acid HIT fold domains with independent active sites that are each sufficient to bind and hydrolyze cognate substrates.

Function:
Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides.

Subunit:
Homodimer. Associates with components of the exosome multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts with NDOR1.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Detected in liver, brain, kidney, testis and prostate.

Similarity:
Belongs to the HIT family.

DCPS,清道夫脱帽酶/热休克蛋白1抗体Database links:
UniProtKB/Swiss-Prot: Q96C86.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.